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human nuclear factor erythroid 2 related factor 2 nrf2 elisa kit  (Cusabio)


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    Cusabio human nuclear factor erythroid 2 related factor 2 nrf2 elisa kit
    Human Nuclear Factor Erythroid 2 Related Factor 2 Nrf2 Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+nrf2+elisa+kit/Human+Nuclear+factor+erythroid+2-related+factor+2(NFE2L2)+ELISA+kit/10__1155_slash_2024_slash_9943747-48-0-22
    Average 93 stars, based on 7 article reviews
    human nuclear factor erythroid 2 related factor 2 nrf2 elisa kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts
    Article Snippet: Dulbecco's modified Eagle medium (DMEM), fusion medium (FM), newborn calf serum, phosphate‐buffered saline (PBS), 1 × 10 5 U/L penicillin and 100 mg/L streptomycin, and Lipofectamine ® RNAiMAX Reagent were purchased from GIBCO Life Technologies Company. .. Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd. .. Lactobacillus delbrueckii bulgaricus was activated, and the activated bacterial strain was inoculated into de Man, Rogosa and Sharpe (MRS) medium and cultured in an oscillator (Qilinbeier, China) at 37°C.

    Reverse Transcription:

    Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts
    Article Snippet: Dulbecco's modified Eagle medium (DMEM), fusion medium (FM), newborn calf serum, phosphate‐buffered saline (PBS), 1 × 10 5 U/L penicillin and 100 mg/L streptomycin, and Lipofectamine ® RNAiMAX Reagent were purchased from GIBCO Life Technologies Company. .. Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd. .. Lactobacillus delbrueckii bulgaricus was activated, and the activated bacterial strain was inoculated into de Man, Rogosa and Sharpe (MRS) medium and cultured in an oscillator (Qilinbeier, China) at 37°C.

    Real-time Polymerase Chain Reaction:

    Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts
    Article Snippet: Dulbecco's modified Eagle medium (DMEM), fusion medium (FM), newborn calf serum, phosphate‐buffered saline (PBS), 1 × 10 5 U/L penicillin and 100 mg/L streptomycin, and Lipofectamine ® RNAiMAX Reagent were purchased from GIBCO Life Technologies Company. .. Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd. .. Lactobacillus delbrueckii bulgaricus was activated, and the activated bacterial strain was inoculated into de Man, Rogosa and Sharpe (MRS) medium and cultured in an oscillator (Qilinbeier, China) at 37°C.

    Malondialdehyde MDA Assay:

    Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts
    Article Snippet: Dulbecco's modified Eagle medium (DMEM), fusion medium (FM), newborn calf serum, phosphate‐buffered saline (PBS), 1 × 10 5 U/L penicillin and 100 mg/L streptomycin, and Lipofectamine ® RNAiMAX Reagent were purchased from GIBCO Life Technologies Company. .. Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd. .. Lactobacillus delbrueckii bulgaricus was activated, and the activated bacterial strain was inoculated into de Man, Rogosa and Sharpe (MRS) medium and cultured in an oscillator (Qilinbeier, China) at 37°C.

    Radio Immunoprecipitation:

    Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts
    Article Snippet: Dulbecco's modified Eagle medium (DMEM), fusion medium (FM), newborn calf serum, phosphate‐buffered saline (PBS), 1 × 10 5 U/L penicillin and 100 mg/L streptomycin, and Lipofectamine ® RNAiMAX Reagent were purchased from GIBCO Life Technologies Company. .. Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd. .. Lactobacillus delbrueckii bulgaricus was activated, and the activated bacterial strain was inoculated into de Man, Rogosa and Sharpe (MRS) medium and cultured in an oscillator (Qilinbeier, China) at 37°C.

    Lysis:

    Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts
    Article Snippet: Dulbecco's modified Eagle medium (DMEM), fusion medium (FM), newborn calf serum, phosphate‐buffered saline (PBS), 1 × 10 5 U/L penicillin and 100 mg/L streptomycin, and Lipofectamine ® RNAiMAX Reagent were purchased from GIBCO Life Technologies Company. .. Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd. .. Lactobacillus delbrueckii bulgaricus was activated, and the activated bacterial strain was inoculated into de Man, Rogosa and Sharpe (MRS) medium and cultured in an oscillator (Qilinbeier, China) at 37°C.

    Cell Counting:

    Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts
    Article Snippet: Dulbecco's modified Eagle medium (DMEM), fusion medium (FM), newborn calf serum, phosphate‐buffered saline (PBS), 1 × 10 5 U/L penicillin and 100 mg/L streptomycin, and Lipofectamine ® RNAiMAX Reagent were purchased from GIBCO Life Technologies Company. .. Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd. .. Lactobacillus delbrueckii bulgaricus was activated, and the activated bacterial strain was inoculated into de Man, Rogosa and Sharpe (MRS) medium and cultured in an oscillator (Qilinbeier, China) at 37°C.

    Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.
    Article Snippet: .. PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 105 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio (https://www.cusabio.cn/, accessed on 23 March 2022), Wuhan, China. ..

    Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts
    Article Snippet: .. PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 10 5 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio ( https://www.cusabio.cn/ , accessed on 23 March 2022), Wuhan, China. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts
    Article Snippet: Dulbecco's modified Eagle medium (DMEM), fusion medium (FM), newborn calf serum, phosphate‐buffered saline (PBS), 1 × 10 5 U/L penicillin and 100 mg/L streptomycin, and Lipofectamine ® RNAiMAX Reagent were purchased from GIBCO Life Technologies Company. .. Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd. .. Lactobacillus delbrueckii bulgaricus was activated, and the activated bacterial strain was inoculated into de Man, Rogosa and Sharpe (MRS) medium and cultured in an oscillator (Qilinbeier, China) at 37°C.

    Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.
    Article Snippet: .. PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 105 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio (https://www.cusabio.cn/, accessed on 23 March 2022), Wuhan, China. ..

    Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts
    Article Snippet: .. PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 10 5 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio ( https://www.cusabio.cn/ , accessed on 23 March 2022), Wuhan, China. ..

    Western Blot:

    Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.
    Article Snippet: .. PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 105 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio (https://www.cusabio.cn/, accessed on 23 March 2022), Wuhan, China. ..

    Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts
    Article Snippet: .. PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 10 5 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio ( https://www.cusabio.cn/ , accessed on 23 March 2022), Wuhan, China. ..

    CCK-8 Assay:

    Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.
    Article Snippet: .. PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 105 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio (https://www.cusabio.cn/, accessed on 23 March 2022), Wuhan, China. ..

    Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts
    Article Snippet: .. PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 10 5 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio ( https://www.cusabio.cn/ , accessed on 23 March 2022), Wuhan, China. ..



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    Fig. 1 Concentration of ferroptosis and OS-related biomarkers in SCZ and HC groups. Panels (a) through (j) depict the concentration levels of the following biomarkers in both groups: a glutathione peroxidase 4 (GPX4), b glutathione (GSH), c iron, d transferrin (TF), e sirtuin 1 (SIRT1), f nuclear factor erythroid 2-related factor 2 (Nrf2), g acyl-CoA synthetase long-chain family member 4 (ACSL4), h ferritin (FE), i superoxide dismutase (SOD), and j malondialdehyde (MDA). **P < 0.01; ***P < 0.001.

    Journal: Schizophrenia (Heidelberg, Germany)

    Article Title: The role of ferroptosis and oxidative stress in cognitive deficits among chronic schizophrenia patients: a multicenter investigation.

    doi: 10.1038/s41537-025-00555-8

    Figure Lengend Snippet: Fig. 1 Concentration of ferroptosis and OS-related biomarkers in SCZ and HC groups. Panels (a) through (j) depict the concentration levels of the following biomarkers in both groups: a glutathione peroxidase 4 (GPX4), b glutathione (GSH), c iron, d transferrin (TF), e sirtuin 1 (SIRT1), f nuclear factor erythroid 2-related factor 2 (Nrf2), g acyl-CoA synthetase long-chain family member 4 (ACSL4), h ferritin (FE), i superoxide dismutase (SOD), and j malondialdehyde (MDA). **P < 0.01; ***P < 0.001.

    Article Snippet: Plasma levels of GPX4 (EK14343, Signalway Antibody, Greenbelt, MD, USA), ACSL4 (ELK8993, ELK Biotechnology Co., Ltd., Wuhan, China), TF, FE, SIRT1, and Nrf2 (E-EL-H6028, E-EL-H0168, E-EL-H1546, E-EL-H1564; Elabscience Biotechnology Co., Ltd., Wuhan, China) were quantified using the enzyme-linked immunosorbent assay (ELISA) kit as per manufacturer instructions.

    Techniques: Concentration Assay